Anti-Vimentin Antibody

Raised in rabbit and affinity-purified, this polyclonal reagent from Antibodies.com is supplied as a 50 µL liquid aliquot and is validated for use in Western blot (WB) and Immunohistochemistry (IHC). The unconjugated IgG antibody specifically detects endogenous Vimentin, a ~53 kDa class-III intermediate filament protein that serves as a canonical marker of mesenchymal cells, fibroblasts, and cells undergoing epithelial-to-mesenchymal transition. Recommended starting dilutions are 1:500–1:1,000 for WB and 1:50–1:200 for IHC, with typical working concentrations around 1 mg/mL in a glycerol-containing storage buffer shipped on blue ice and intended for long-term storage at –20 °C (avoid repeated freeze-thaw cycles). Reactivity has been confirmed in Human, Mouse and Rat samples, making the reagent well suited to cross-species studies of cytoskeletal remodeling, tumor invasion, EMT signaling, stem-cell biology and wound-healing research. Each lot is backed by the Antibodies.com 100% quality guarantee and is supported by PhD-level technical assistance from the manufacturer.