Anti-Vimentin Antibody

This rabbit polyclonal antibody targets vimentin, a 57 kDa type III intermediate filament protein that maintains cytoskeletal integrity in mesenchymal cells and serves as a canonical marker for epithelial-to-mesenchymal transition (EMT). Produced by Antibodies.com, the reagent is raised in rabbit and supplied as an unconjugated, polyclonal preparation at 100 µl per vial, ready for standard immunodetection workflows.

The antibody has been validated for Western blot analysis of the ~57 kDa vimentin band, immunohistochemistry on formalin-fixed tissue sections, immunofluorescence imaging of the intermediate filament network in cultured cells, and ELISA-based assays. Its reactivity profile is specific to human vimentin, supporting studies of carcinoma progression, sarcoma characterization, fibroblast biology, wound healing, fibrosis, and stem cell differentiation research.

Aliquot upon arrival and store refrigerated to preserve antibody activity across multiple experiments. As an unconjugated reagent, this antibody can be paired with a broad selection of secondary detection reagents and fluorophore conjugates, enabling flexible assay design.