Anti-Vimentin Antibody

Vimentin is a 57 kDa type III intermediate filament protein that serves as a canonical marker of mesenchymal origin and is widely used to track epithelial-to-mesenchymal transition in cancer, fibrosis, and developmental biology studies. This unconjugated Mouse IgG1 monoclonal reagent from Antibodies.com detects the target in human, mouse, and rat samples, supporting immunological work across species boundaries.

Recommended applications include Western blot for protein-level expression profiling, immunocytochemistry on cultured cells, immunohistochemistry on tissue sections, and flow cytometry for intracellular staining. The antibody is supplied as 100 µl of liquid in 1×TBS (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, providing a ready-to-dilute format compatible with standard assay buffers.

Because vimentin is retained or upregulated in many carcinomas undergoing EMT and functions as a routine identifier of fibroblasts and stromal compartments, this reagent is commonly deployed in tumor invasion studies, wound healing models, and stem cell characterization. Researchers should titrate empirically for their platform, since optimal dilutions differ between immunofluorescence, chromogenic IHC, and flow cytometry workflows.