Anti-VIM Antibody

Distributed through Antibodies.com under the Primary Antibodies category, this affinity-purified Rabbit polyclonal reagent was raised against the VIM (vimentin) target and is supplied as a 50 µl liquid aliquot at 1 mg/ml in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The unconjugated antibody is validated for Western blot (WB) and Immunofluorescence (IF) workflows, with recommended working dilutions of WB 1:500–1:1000 and IF 1:100–1:200, and it is raised in a Rabbit host with polyclonal clonality (IgG isotype), detecting endogenous levels of total VIM protein across Human, Mouse and Rat samples (with additional reactivity to monkey confirmed on the supplier's product sheet). The immunogen corresponds to a peptide sequence around residues 452–456 (G-Q-V-I-N) of human Vimentin, and the antibody is purified by affinity chromatography using an epitope-specific peptide before being dispensed in ready-to-use aliquots that should be stored at –20 °C for long-term stability (stable for up to one year as supplied). Each lot ships with HRP-, FITC-, biotin- and AP-conjugated Goat anti-Rabbit IgG secondaries available separately for downstream detection, and the reagent is intended for research use only — not for diagnostic or therapeutic applications. Researchers handling mesenchymal, fibroblast or cytoskeletal targets will appreciate the broad species reactivity, the clean unconjugated format that pairs with the wide range of compatible Rabbit IgG secondaries offered on Antibodies.com, and the standardized 1 mg/ml formulation that simplifies dilution calculations across WB and IF experiments.