Anti-VIM Antibody

Vimentin (VIM) is a 57 kDa type III intermediate filament protein that anchors and supports cytoplasmic organelles while preserving cell shape and mechanical resilience in mesenchymal-derived cells. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of ready-to-use formulation and has been validated for Western blot and immunofluorescence workflows across human, mouse, and rat sample preparations.

In routine laboratory practice, the antibody serves as a reliable marker for distinguishing mesenchymal lineages from epithelial counterparts and is widely applied in studies of epithelial-mesenchymal transition, wound healing, fibrosis, and tumor invasion. Researchers commonly pair it with cytokeratin staining in co-localization experiments, and it is frequently used to characterize fibroblast cultures, assess stromal content in tumor sections, and monitor cytoskeletal reorganization in response to chemical or mechanical stimuli. Store the vial at -20 °C in small aliquots to limit freeze-thaw cycles, and protect from light to preserve activity through repeated use.