Anti-VIM Antibody

Manufactured by Antibodies.com, this affinity-purified reagent is a rabbit polyclonal antibody raised against the C-terminal region of human vimentin (VIM), designed to detect endogenous levels of the target protein across a wide range of experimental workflows. Supplied as a 50 µl liquid formulation in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide at a working concentration of 1 mg/mL, the unconjugated primary antibody is validated for ELISA, Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF) and immunohistochemistry (IHC). The antibody has been confirmed to react with human, mouse and rat samples, and its polyclonal nature allows recognition of multiple epitopes on vimentin, supporting robust detection in both cell lysate and tissue-section preparations. Shipped on cold packs at 4 °C, the vial should be aliquoted upon receipt and stored long-term at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Typical published validation data from Antibodies.com illustrate detection of endogenous VIM in HepG2 whole-cell lysates alongside a panel of additional lines including AD293, K562 and mouse brain, confirming broad utility for cytoskeletal and mesenchymal-marker research. Each lot is produced under the supplier's 100 % guarantee and price-match terms, with lead times of 6–9 business days for orders dispatched from St. Louis, MO.