Anti-Villin Antibody

Studying the epithelial cytoskeleton, intestinal brush border assembly, and gastrointestinal tumor lineage requires a reliable marker for differentiated enterocytes. This unconjugated rabbit polyclonal antibody from Antibodies.com targets Villin (VIL1), the calcium-regulated actin-bundling protein that organizes the microvillar core of intestinal and renal proximal tubule cells, and is supplied as a 100 µl vial of antiserum.

Validated on human samples for Western blot, immunohistochemistry, and immunofluorescence, the reagent labels differentiated epithelial cells and is widely used in research to confirm gastrointestinal or renal epithelial origin in tumor profiling, often in panels alongside cytokeratins and CDX2. The polyclonal format increases the chance of recognizing multiple epitopes on VIL1, which is helpful when probing denatured lysates or formalin-fixed, paraffin-embedded sections.

For handling, aliquot upon arrival and store at -20°C; avoid repeated freeze-thaw cycles to preserve activity. The antibody is specifically validated for reactivity with human Villin and is provided unconjugated, allowing flexible secondary antibody selection for fluorescence, chromogenic, or chemiluminescent detection systems in research use only workflows.