Anti-VEGFR2/Flk-1 (P957) Antibody

Phospho-specific detection of VEGFR2 at tyrosine 957 (Y957) is the focus of this rabbit polyclonal reagent from Antibodies.com, supplied as 100 µl of unconjugated antibody at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2. The antibody selectively recognizes VEGFR2 only when phosphorylated at the Y957 autophosphorylation site, a residue implicated in VEGF-driven mitogenic signaling and endothelial cell proliferation, making it a targeted tool for angiogenesis research. Validated for immunohistochemistry (IHC), the reagent reacts with both human and mouse tissue samples, supporting studies in tumor vasculature, wound healing models, and developmental biology where VEGFR2 activation status is the experimental variable of interest. Polyclonal antibodies raised against a phosphopeptide at this site offer broader epitope coverage than monoclonal alternatives, which can be advantageous when working with formalin-fixed, paraffin-embedded (FFPE) sections where phospho-epitope preservation varies from block to block. The unconjugated format permits flexible pairing with user-selected secondary detection reagents, including HRP, biotin, or fluorophore conjugates matched to the chosen IHC workflow.