Anti-VEGFR2/Flk-1 (F945) Antibody

This unconjugated rabbit polyclonal reagent targets the F945 epitope region of VEGFR2 (also called KDR/Flk-1), a type II receptor tyrosine kinase that acts as the principal signal-transducing receptor for vascular endothelial growth factor on endothelial cells. Supplied by Antibodies.com as a 100 µl volume at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide (pH approximately 7.2), the antibody is validated for Western blot, immunohistochemistry, and immunofluorescence workflows on human, mouse, and rat samples.

Researchers use the reagent to map VEGFR2 expression in tumor vasculature, characterize angiogenic signaling in primary endothelial cell models, and probe neovascularization in tissue sections from cancer xenografts, retinal neovascularization models, and ischemic tissue repair studies. Because VEGFR2 phosphorylation drives downstream MAPK and PI3K/Akt cascades in response to VEGF-A binding, total receptor detection supports studies of pathway dysregulation in conditions where angiogenesis is pathologically altered, including solid tumor growth and diabetic microvascular complications.

The polyclonal format recognizes multiple epitopes across the receptor's extracellular and intracellular domains, which is useful when assessing overall receptor abundance rather than a single phospho-site. Aliquot upon receipt to limit freeze-thaw cycles, store refrigerated for short-term use, and protect from prolonged light exposure. The azide-preserved solution is intended for research use only.