Anti-VEGF (11B5) Antibody

Vascular Endothelial Growth Factor (VEGF) drives angiogenesis and increases vascular permeability, making it a focal target in oncology, ophthalmology, and vascular biology research. Antibodies.com offers this monoclonal antibody (clone 11B5) raised in mouse tissue culture, supplied as 1 ml of unconjugated supernatant for direct use in western blot and immunohistochemistry workflows on both frozen and paraffin-embedded tissue.

Several practical points are worth noting. The IgM,k isotype is uncommon among research-grade monoclonals, which are predominantly IgG; this unusual class affects secondary reagent selection, since anti-IgM secondary antibodies are required for detection. The unconjugated format keeps the antibody flexible for custom labeling or pairing with user-preferred detection chemistries, and researchers running western blots typically load tens of micrograms of total protein per lane to visualize VEGF at endogenous expression levels.

For sample handling, briefly centrifuge the vial before opening to recover the full 1 ml volume and protect the reagent from light during working use. Aliquoting after first thaw is recommended to limit freeze-thaw cycles and preserve binding activity across repeated experiments. Investigators studying tumor neovascularization, retinal disease models, or chronic wound healing will find this clone useful for tracking VEGF protein expression by western blot and immunohistochemistry in parallel.