Anti-VE-Cadherin Antibody

VE-Cadherin (CDH5) is a calcium-dependent transmembrane glycoprotein that forms adherens junctions between adjacent vascular endothelial cells, where it mediates homophilic adhesion and couples the actin cytoskeleton to the plasma membrane via cytoplasmic catenins. This rabbit polyclonal reagent from Antibodies.com was raised against the human protein and shows reactivity with both human and mouse samples, supporting comparative studies of vascular biology, angiogenesis, and endothelial barrier function across these species.

For Western blot applications, the antibody is supplied unconjugated at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, packaged as a 50 µl vial. This format permits direct immunodetection on nitrocellulose or PVDF membranes after SDS-PAGE separation of endothelial lysates, with working dilutions commonly titrated between 1:500 and 1:2000 depending on target abundance. The glycerol-containing buffer enables storage at -20°C, and aliquoting into single-use volumes upon receipt is recommended to minimize repeated freeze-thaw cycles and preserve binding activity over extended use.