Anti-VCL Antibody

This unconjugated rabbit polyclonal reagent from Antibodies.com targets vinculin (VCL), a 124 kDa cytoskeletal protein concentrated at focal adhesions and adherens junctions where it couples actin filaments to the cell membrane and reinforces mechanical tension between neighboring cells. The reagent is supplied as 100 µL of polyclonal antiserum raised in rabbit and is validated for Western blot and immunohistochemistry workflows. It recognizes vinculin isoforms from human, mouse, and rat samples, making it a practical choice for comparative studies of cell adhesion, migration, and epithelial-mesenchymal transition in tissue sections and lysates. Researchers commonly apply it to investigate focal adhesion remodeling in cancer cell lines, integrin signaling in cardiovascular models, and cytoskeletal organization in neuronal cultures. Working dilutions should be titrated empirically against the chosen detection chemistry.