Anti-VAPA Antibody

Manufactured by Antibodies.com as a Rabbit polyclonal reagent, this primary antibody targets VAPA (VAMP-associated protein A), a 33 kDa membrane-associated protein implicated in vesicle trafficking and ER–organelle tethering. The immunogen corresponds to a region within amino acids 29 and 92 of human VAPA, and the antibody is supplied as an unconjugated, antigen-affinity purified preparation in a 100 µl volume. It is formulated in 0.1 M Tris-buffered saline (pH 7.0) containing 10% glycerol and 0.01% thimerosal as a preservative, and is shipped at 4 °C; for long-term storage, aliquot and keep at –20 °C, avoiding repeated freeze/thaw cycles. The reagent is recommended for Western blotting at a working dilution of approximately 1:1000 on whole-cell lysates (validated on H1299 cells separated by 12% SDS-PAGE) and for immunohistochemistry on tissue sections, supporting both expression and localisation studies of endogenous VAPA. Cross-reactivity has been confirmed in Human and Mouse samples, making the antibody suitable for comparative work between the two species. As an IgG-class polyclonal raised in Rabbit, the reagent offers broad epitope coverage of the target, while its unconjugated format allows flexibility with a wide range of secondary-detection chemistries, including HRP, alkaline phosphatase and fluorescent labels. Each lot is affinity-purified to reduce nonspecific background, and the product is intended for research use only. For laboratories running routine immunoblotting and IHC workflows on VAPA-expressing cells or tissue, this Antibodies.com polyclonal provides a well-characterised option backed by clearly reported validation data.