Anti-V5 Tag Antibody [V531]

Offered through the Antibodies.com catalogue, this Mouse monoclonal reagent is the clone V531, raised against a synthetic peptide spanning amino acid residues 95–108 (sequence GKPIPNPLLGLDST) of the RNA polymerase alpha subunit of Simian virus 5 (SV5), conjugated to KLH for immunization. Produced in the Sp2/0-Ag14 myeloma line and purified by Protein G affinity chromatography, the antibody is supplied unconjugated as a 500 µg lyophilised preparation in 0.01 M Phosphate Buffered Saline, pH 7.2, containing 0.05% Sodium Azide; final concentration is reconstitution-dependent. It belongs to the IgG1 subclass and is validated for Western Blot (WB) detection of V5-tagged recombinant proteins in human samples, making it a reliable tool for researchers working with tagged constructs. The reagent is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, with freeze/thaw cycles avoided to preserve activity. For laboratory research use only; not intended for diagnostic or therapeutic applications.