Anti-V5 Tag Antibody [KT121]

The KT121 clone is a mouse monoclonal antibody that specifically recognizes the 14-amino-acid V5 epitope (GKPIPNPLLGLDST) derived from paramyxovirus P/V proteins. Supplied by Antibodies.com as a 200 µg unconjugated IgG3 liquid formulation, this reagent is validated for Western blot and ELISA detection of V5-tagged recombinant fusion proteins. It binds the linear V5 sequence commonly placed at the N- or C-terminus of engineered constructs to enable expression verification, tracking, and immunoprecipitation support experiments.

For optimal stability, aliquot and store at -20 °C, avoiding repeated freeze-thaw cycles. Suggested working dilutions range from 1:1000 to 1:5000 for Western blot applications, with ELISA protocols optimized per individual assay design.