Anti-V5 Tag Antibody [KT121]

The Antibodies.com Anti-V5 Tag Antibody [KT121] is a mouse monoclonal IgG3 reagent that recognizes the V5 epitope (GKPIPNPLLGLDST) derived from simian virus 5, enabling consistent detection of recombinant fusion proteins in heterologous expression systems. Supplied as a 100 µg liquid aliquot, the unconjugated antibody supports western blot and ELISA workflows where an anti-tag primary reagent is needed.

This clone has been used to validate V5-tagged constructs in cell lysates and purified preparations, binding the linear epitope after standard SDS-PAGE transfer. For long-term storage, aliquot the antibody and freeze at -20 degrees C, avoiding repeated freeze-thaw cycles that can reduce binding activity. Researchers commonly pair this primary reagent with HRP- or fluorophore-conjugated anti-mouse secondary antibodies for chemiluminescent western blot imaging or plate-based ELISA detection of tagged recombinant proteins.