Anti-V5 Tag Antibody

The Antibodies.com anti-V5 Tag antibody is a mouse monoclonal IgG1 clone that recognizes the 14-amino-acid V5 epitope (GKPIPNPLLGLDST) derived from the P and V proteins of simian virus 5. This short peptide tag is widely fused to recombinant proteins expressed in mammalian, insect, and bacterial hosts, allowing consistent detection with a single reagent. The unconjugated antibody is supplied as a 100 µg liquid formulation in a vial, ready for direct use in Western blot, immunoprecipitation, and immunofluorescence workflows without further dilution. For long-term storage, aliquot the antibody and freeze at -20°C to preserve activity across multiple freeze-thaw cycles. The reagent is validated for research use only and performs reliably with common V5-fusion expression constructs, including pcDNA3.1-V5 and pCI-neo-V5 vectors used in transfected cell lines.