Anti-V-type proton ATPase subunit G 1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets the G1 peripheral stalk subunit of vacuolar-type H+ ATPase (V-ATPase), a multi-subunit rotary proton pump that acidifies intracellular organelles and selected plasma membrane domains. The unconjugated reagent is supplied as a 100 µg aliquot and is validated for immunoblotting (WB) and immunohistochemistry (IHC) on formalin-fixed tissue sections.

Because the G1 isoform contributes to the peripheral stator that couples ATP hydrolysis to proton translocation, the reagent is commonly used to map V-ATPase distribution in renal tubule epithelium, osteoclast ruffled borders, and neuronal synaptic vesicle pools. Reactivity is reported with human, mouse, and rat samples, supporting work across cell line panels and rodent models relevant to pH homeostasis, bone resorption, and renal acid-base handling.

For western blot, denatured lysate loadings in the low-microgram range yield a band consistent with the expected molecular weight of the G1 subunit; for IHC, citrate-based antigen retrieval is recommended for paraffin-embedded sections. Store refrigerated and protect from light to preserve antibody activity across the working shelf life.