Anti-USP25 Antibody

Produced in rabbit against the deubiquitinase USP25, this unconjugated polyclonal antibody from Antibodies.com is supplied as a 200 µl aliquot and validated for Western blot, immunohistochemistry, and immunoprecipitation on human, mouse, and rat samples. USP25 (Ubiquitin-Specific Peptidase 25) is a cysteine-dependent protease that removes ubiquitin from substrate proteins and modulates pathways tied to innate immunity, ER-associated degradation, and proteasome signaling. The enzyme contains tandem UBP-type catalytic domains together with a ubiquitin-associated (UBA) domain, and the full-length protein migrates near the ~122 kDa marker on reducing SDS-PAGE, providing a useful size reference when interpreting WB lanes. Researchers use this reagent to study TRAF3 deubiquitination, type I interferon regulation, and IL-17-driven inflammation, as well as the enzyme's documented links to trisomy 21 phenotypes arising from its chromosomal location. The polyclonal format recognises multiple epitopes on USP25, which improves tolerance for partial proteolysis or fixation-induced masking during IP and IHC workflows. The unconjugated IgG isotype supports downstream amine-reactive labelling in custom conjugation workflows, giving flexibility to attach reporter enzymes or fluorophores as required by the chosen detection method.