Anti-USP16 Antibody

USP16 (Ubiquitin Specific Peptidase 16) is a chromatin-associated deubiquitinating enzyme that removes mono-ubiquitin from histone H2A at lysine 119, the modification deposited by the Polycomb repressive complex 1 and read by Polycomb-mediated gene silencing machinery. By modulating H2AK119ub levels at promoter and enhancer regions, USP16 influences transcription, the DNA damage response, cell cycle progression, and lineage commitment in hematopoietic and neural stem cells. Altered USP16 expression and activity have been reported in acute myeloid leukemia, multiple myeloma, breast carcinoma, and lung adenocarcinoma, and the gene maps within the Down syndrome critical region of chromosome 21, where elevated dosage contributes to neurodevelopmental and craniofacial phenotypes.

Antibodies.com offers this rabbit polyclonal Anti-USP16 Antibody as an unconjugated reagent supplied in a 100 µl vial. The antibody was raised in rabbit, is affinity-purified, and has been validated for western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows. It detects endogenous USP16 in human, mouse, and rat samples, making it useful for cross-species studies of chromatin biology, ubiquitin pathway analysis, and disease model characterization in fixed cells, frozen sections, and freshly prepared lysates.

Practical applications include confirming CRISPR-Cas9 or RNAi knock-down efficiency at the protein level, mapping sub-nuclear localization of USP16 in interphase and mitotic cells, and immunoprecipitating USP16-containing chromatin complexes for downstream mass spectrometry. The unconjugated format gives the end user freedom to pair the antibody with HRP, fluorophore, or biotin secondary reagents, while the polyclonal nature provides recognition of multiple epitopes across the target protein.