Anti-UMOD Antibody

Renal tubular research into uromodulin biology requires a rigorously validated detection reagent, and this unconjugated rabbit polyclonal IgG from Antibodies.com is affinity-purified against the UMOD target. Uromodulin, also called Tamm-Horsfall protein, is the most abundant glycoprotein in normal urine and is produced exclusively by epithelial cells of the thick ascending limb of Henle and the early distal convoluted tubule, which makes it a useful marker for studying tubular function, electrolyte handling, and inherited disorders such as autosomal dominant tubulointerstitial kidney disease (ADTKD-UMOD).

Validated for Western Blot, the antibody recognises endogenous UMOD in both human and mouse samples, making it suitable for parallel studies in patient-derived material and murine models of cystic kidney disease, nephrolithiasis, and salt-sensitive hypertension. Researchers typically resolve whole-cell or tissue lysates by SDS-PAGE, transfer to nitrocellulose or PVDF, and probe at empirically calibrated dilutions; expected signal appears in the 70–90 kDa region, reflecting the mature glycosylated form of the protein. The reagent is supplied as a 50 µL liquid aliquot of polyclonal serum, unconjugated and compatible with standard HRP- or fluorophore-conjugated secondary antibodies for immunoblotting workflows.