Anti-UHRF1 (ChIP Grade) Antibody

UHRF1 (Ubiquitin-like with PHD and RING finger domains 1) is a nuclear epigenetic regulator that maintains DNA methylation patterns through cell division. The protein reads hemimethylated CpG sites generated during DNA replication and recruits the maintenance methyltransferase DNMT1 to propagate the methylation mark on the newly synthesized strand. Beyond its canonical role in methylation inheritance, UHRF1 contributes to histone ubiquitination, chromatin compaction, and the DNA damage response, and its overexpression in multiple solid tumors has made it a subject of active investigation in cancer epigenetics.

This ChIP-validated reagent from Antibodies.com is a rabbit polyclonal antibody raised against human UHRF1, unconjugated and supplied at 1 mg/mL in a 100 µL volume formulated in PBS with 0.1% sodium azide and 50% glycerol. The product is validated for western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation workflows on human samples, with the ChIP-grade designation reflecting confirmed performance in chromatin pull-down assays used to map UHRF1 occupancy at target promoters and pericentromeric regions. The 50% glycerol buffer permits storage at -20°C with minimal freeze-thaw loss, supporting repeated aliquoting across multiple experiments.