Anti-UBF1 Antibody

This rabbit polyclonal antibody from Antibodies.com targets UBF1 (Upstream Binding Factor 1), a nucleolar transcription factor essential for RNA Polymerase I-driven transcription of ribosomal RNA genes. UBF1 binds to the upstream control element and promoter of the rDNA repeat, facilitating pre-initiation complex assembly and modulating chromatin architecture at active nucleolar organizer regions.

Supplied as 100 µL of unconjugated antisera, the reagent is validated for Western blot and immunohistochemistry workflows, with reactivity confirmed against human, mouse, and rat samples. The polyclonal format provides recognition of multiple epitopes across the UBF1 protein, useful for detecting endogenous expression in cell lysates by Western blot and for visualizing nucleolar localization in fixed tissue sections by IHC.

UBF1 is widely studied in the context of ribosome biogenesis, cell growth regulation, and nucleolar stress responses, with altered expression reported in several cancer models. Researchers investigating rRNA transcription, nucleolar structure, or proliferation-associated signaling pathways can use this antibody to monitor UBF1 levels across experimental conditions. The unconjugated format allows flexible pairing with custom fluorophore or enzyme conjugations. Store refrigerated and avoid repeated freeze-thaw cycles to preserve antibody activity.