Anti-UBE4A Antibody

Among ubiquitin pathway research tools, the UBE4A-targeting polyclonal antibody from Antibodies.com offers a broad-epitope recognition approach for Western blot (WB) detection across Human, Mouse, and Rat sample types. UBE4A encodes the E4 ubiquitin conjugation enzyme, functioning as an E3-E4 hybrid ligase that assembles multi-ubiquitin chains on substrate proteins during the ubiquitin-proteasome degradation cycle, with documented involvement in DNA damage response, p53 polyubiquitination, and chromatin remodeling processes.

Supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, this 100 µl vial format reagent is raised in a Rabbit host to expand the recognition footprint across the target protein. Polyclonal preparations engage multiple non-overlapping determinants, which can strengthen signal capture when endogenous UBE4A levels are modest or when probing orthologous proteins within the tested species panel.

Investigators studying protein turnover, post-translational modification cascades, ubiquitin-dependent signaling, or DNA repair pathways commonly deploy this unconjugated antibody to confirm UBE4A expression in cell lysates or tissue homogenates resolved on denaturing SDS-PAGE gels and transferred onto nitrocellulose or PVDF membranes.