Anti-UBE3C Antibody

This rabbit polyclonal antibody targets UBE3C, a HECT-domain E3 ubiquitin ligase that drives substrate ubiquitination for proteasomal turnover and participates in endoplasmic reticulum-associated degradation. The reagent is supplied by Antibodies.com as an unconjugated, liquid-format immunogen affinity-purified preparation in a 100 µL vial, suitable for Western blot detection of endogenous UBE3C in human cell lysates and for immunofluorescence localization studies.

UBE3C catalyzes the addition of ubiquitin chains on substrate lysine residues through its C-terminal HECT catalytic domain and contributes to quality-control pathways that clear misfolded proteins. Researchers investigating cancer cell proteostasis, stress-response signaling, and the ubiquitin-proteasome system use this reagent to probe expression changes and subcellular distribution of the ligase.

For storage, the antibody should be aliquoted upon arrival and stored at -20 degrees C, with freeze-thaw cycles minimized to preserve binding activity. Working dilutions should be optimized empirically for each application.