Anti-UBE3A Antibody

This polyclonal antibody targets UBE3A, the HECT-domain E3 ubiquitin ligase encoded within the 15q11-q13 imprinted locus that is strongly implicated in Angelman syndrome neurodevelopmental research. Raised in rabbit and supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the reagent is unconjugated and validated for Western blot (WB) and immunohistochemistry (IHC) on human, mouse, and rat samples.

Researchers commonly deploy this Antibodies.com reagent to interrogate maternal allele expression in neurons, where the paternal UBE3A copy is silenced by the UBE3A-ATS antisense transcript. The 100 µl vial format suits typical laboratory workflows spanning cell culture lysates, frozen brain tissue sections, and patient-derived iPSC differentiation models used to study imprinting defects and proteasomal substrate turnover. The glycerol-containing buffer with sodium azide as preservative supports stable handling during routine use, including SDS-PAGE membrane probing and IHC antigen retrieval protocols.