Anti-UBE2N Antibody

Raised in rabbit against the human UBE2N sequence, this polyclonal IgG reagent from Antibodies.com is calibrated for the ~17 kDa ubiquitin-conjugating E2 enzyme synonymous with UBC13. The antibody ships as 100 µl of unconjugated immunoglobulin, leaving secondary choice open: HRP, fluorescent, or biotinylated reporters can be paired downstream depending on the assay design.

UBE2N is the catalytic half of the UEV1-UBE2N heterodimer and drives K63-linked polyubiquitin chain assembly on target proteins. Published roles include translesion DNA synthesis past replication-blocking lesions, post-replication repair of stalled forks, NF-κB pathway activation downstream of TNF and IL-1 receptors, and TRAF6-driven innate immune signaling. Cross-reactivity with Human and Rat orthologs lets investigators compare chromatin-bound ubiquitination and inflammatory cascade readouts across species in matched whole-cell lysate preparations.

Validation covers Western Blot on reducing SDS-PAGE gels, where the endogenous target resolves near 17 kDa. Storage and handling: keep the vial at -20 °C, aliquot upon first thaw to limit freeze-thaw cycles, avoid prolonged room-temperature exposure, and briefly centrifuge the tube before opening to recover the full liquid volume.