Anti-UBE2I Antibody

SUMOylation depends on a single dedicated E2-conjugating enzyme, UBC9, which is encoded by the UBE2I gene and is responsible for transferring SUMO-1, SUMO-2, and SUMO-3 onto lysine residues of substrate proteins. SUMO modification by this enzyme reshapes DNA damage responses, mitotic progression, nuclear-cytoplasmic trafficking, and transcriptional regulation, making the protein a frequently probed node in cell signalling studies. This polyclonal reagent from Antibodies.com is raised in rabbit and affinity-purified to recognise the human UBE2I sequence, and it is verified for Western blot on whole-cell lysates and for immunohistochemistry on fixed tissue sections. The unconjugated IgG format leaves each lab free to choose its own reporter (HRP, biotin, or a fluorophore) when designing an immunoblot or chromogen-based IHC experiment. Each vial contains 200 µl of working stock, sized for routine membrane probing and standard slide staining without an immediate need to aliquot. Because the target is highly conserved across eukaryotes, this reagent also serves as a useful cross-reactive detection reagent in orthogonal SUMO-pathway screens where UBC9 levels are correlated with SUMO-conjugate accumulation.