Anti-UBE2I Antibody

Raised in rabbit against the SUMO-E2 conjugating enzyme UBE2I (also known as UBC9), this unconjugated polyclonal primary antibody from Antibodies.com detects the human, mouse, and rat protein across western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation workflows. The 100 µl format supplies enough reagent for dozens of mini-gel blots, slide staining experiments, or lysate-clearing immunoprecipitations.

UBE2I operates at the heart of the SUMOylation cascade, catalysing covalent attachment of SUMO-1, SUMO-2, and SUMO-3 onto substrate lysine residues. It modifies well-characterised targets such as p53, c-Jun, IκBα, and RanGAP1, and influences DNA-damage responses, transcription factor activity, nuclear transport, and mitotic progression. The unconjugated IgG format leaves the antibody open for downstream biotin, HRP, or fluorophore conjugation, so the same vial serves chromogenic western blot detection, immunofluorescence localisation of nuclear SUMO-pathway components, and IP-MS interactome studies where SUMO-conjugate remodelling is being monitored after proteasome inhibition or DNA-damage induction.

Recommended starting dilutions are approximately 1:500-1:2000 for western blot on whole-cell lysates and 1:100-1:500 for immunofluorescence on paraformaldehyde-fixed cells. Refrigerate reconstituted stock and keep frozen at -20 °C in working aliquots to limit repeated freeze-thaw cycles of the polyclonal IgG pool. Researchers studying SUMO-pathway biology, post-translational modification mapping, or nuclear-cytoplasmic trafficking can pair this UBE2I reagent with anti-SUMO-1, anti-SUMO-2/3, and anti-RanGAP1 antibodies to dissect modification dynamics inside chromatin-associated complexes.