Anti-UBE2I Antibody

Targeting the SUMO-conjugating enzyme UBE2I, this rabbit polyclonal antibody from Antibodies.com supports multiple research applications including Western blotting, immunohistochemistry, immunocytochemistry, immunofluorescence, immunoprecipitation, and RNA immunoprecipitation. UBE2I, also known as UBC9, is the sole E2 ligase responsible for covalent attachment of SUMO paralogues to substrate proteins and plays essential roles in DNA damage response, cell cycle regulation, and nuclear trafficking. The unconjugated antibody is supplied as 100 µl of a 1 mg/ml stock in phosphate-buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, and is validated for reactivity against human, mouse, and rat samples. Recommended storage at 4°C in this buffered formulation preserves activity for short-term use; aliquoting is advised to avoid repeated freeze-thaw cycles when the reagent is held at -20°C or below for longer-term storage. Researchers studying SUMOylation pathways, post-translational modification dynamics, or UBE2I-dependent substrate conjugation will find this reagent applicable across cell lysate, fixed tissue, and native complex workflows.