Anti-UBE2G1 Antibody

UBE2G1 (Ubiquitin-Conjugating Enzyme E2G 1) is a member of the E2 ubiquitin-conjugating enzyme family that catalyzes the transfer of activated ubiquitin onto substrate proteins destined for proteasomal degradation. This polyclonal reagent from Antibodies.com is raised in rabbit and supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide, at approximately pH 7.3. It has been validated for Western blot detection of endogenous UBE2G1 in human, mouse, and rat sample types.

Functionally, UBE2G1 partners with E3 ubiquitin ligases and participates in endoplasmic reticulum-associated degradation (ERAD), the quality-control pathway that disposes of misfolded or unassembled proteins from the ER membrane. Researchers studying neurodegeneration, cancer cell signaling, viral immune evasion, or general proteostasis frequently rely on this antibody to probe E2 enzyme expression levels and substrate ubiquitination status. The polyclonal format offers recognition of multiple epitopes on the target, which improves detection sensitivity when working with low-abundance endogenous protein across varied tissue and cell-line lysates.

Each vial provides 100 µl of antibody, sufficient for multiple Western blot experiments. For best results, aliquot the preparation upon first use and store refrigerated at 4 degrees C; protect from light and avoid repeated freeze-thaw cycles to preserve binding activity. The sodium azide additive helps maintain sterility during routine benchtop handling. Investigators characterizing apoptosis pathways, cell cycle regulation, or specific E2-E3 ligase partnerships will find this reagent useful when mapping UBE2G1-dependent modification events in basic research settings.