Anti-UBE2E3 Antibody

Provided by Antibodies.com as a 100 µL liquid aliquot in a single vial, this affinity-purified rabbit polyclonal IgG targets UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3), a nuclear E2 enzyme that partners with E3 ligases to transfer ubiquitin onto substrate proteins. UBE2E3 activity is implicated in cell-cycle control, stress signaling, and altered expression across multiple cancer types, making it a relevant target for studies of post-translational protein modification. The unconjugated format leaves secondary reporter choice open, supporting either chemiluminescent or chromogenic readouts on Western blot and immunohistochemistry membranes or tissue sections.

Supplied in a glycerol-based PBS buffer (pH 7.4) with sodium azide preservative, the reagent should be aliquoted upon arrival and stored at -20°C, with repeated freeze-thaw cycles avoided to preserve activity. Validation data confirms reactivity against human and mouse samples, where the antibody detects total endogenous UBE2E3 in cell lysates and paraffin-embedded tissue sections. Researchers investigating ubiquitin ligase activity, proteasome pathway biology, and DNA-damage response pathways can use this reagent to probe UBE2E3 abundance in both in vitro cell culture and in vivo model contexts.