Anti-UBE2D3 Antibody

Recognizing UBE2D3 (Ubiquitin-Conjugating Enzyme E2 D3), a member of the UBC4/UBC5 subfamily of E2 ubiquitin-conjugating enzymes, this rabbit polyclonal antibody from Antibodies.com is validated for Western blot (WB) detection of the endogenous human protein. UBE2D3, also designated UBCH5C, functions as a central E2 carrier that cooperates with RING-type E3 ligases to transfer ubiquitin onto diverse substrates, contributing to proteasomal degradation, cell cycle checkpoint control, DNA damage response pathways, and apoptosis regulation. The unconjugated IgG reagent reacts specifically with human UBE2D3, offering flexibility in choosing secondary antibodies and detection chemistries (HRP, fluorescent, or chromogenic) suited to a particular immunoblotting workflow. Each vial contains 100 µl of polyclonal antibody, sufficient for multiple immunoblotting experiments. For optimal stability, store at -20 °C and aliquot to limit freeze-thaw cycles, with short-term refrigerated storage acceptable during active use. Researchers examining ubiquitination biology, cancer cell signaling, p53 pathway regulation, and protein turnover studies will benefit from reliable detection of UBE2D3 across cell lysates and tissue homogenates.