Anti-UBB Antibody

Raised in rabbit against Ubiquitin B (UBB), this polyclonal reagent from Antibodies.com is supplied as 100 µl of unconjugated IgG in a vial. The immunogen is a recombinant fragment of the human UBB precursor, and the antibody detects endogenous UBB across human, mouse, and rat lysates, supporting comparative work in cell line panels, primary cultures, and tissue homogenates.

Validated for Western blot, the antibody is recommended at working dilutions of 1:500 to 1:2000 in standard blocking buffer with overnight incubation at 4 °C. The product is formulated in phosphate-buffered saline containing glycerol as a cryoprotectant, and the vial should be stored at -20 °C in single-use aliquots to avoid repeated freeze-thaw cycles that erode antibody performance over time. Shipment is on cold packs to preserve activity on arrival.

Because UBB encodes tandem ubiquitin monomers that are cleaved and conjugated to substrate lysine residues via the E1–E2–E3 enzymatic cascade, this primary antibody is applicable to studies of proteasomal degradation, endocytic sorting, DNA repair, autophagy flux, and aggregate clearance. The polyclonal format gives recognition of multiple epitopes on the precursor, which is helpful when analyzing polyubiquitinated substrates where conjugation may partially mask a single site. The reagent is for research use only and supports laboratory work in basic cell biology, neuroscience, and cancer research.