Anti-UAP1 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com is raised against UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1), the terminal enzyme in the hexosamine biosynthetic pathway that produces UDP-GlcNAc, the nucleotide-sugar donor used by the cell for N-linked and O-linked protein glycosylation. Supplied as 100 µl of unconjugated antibody, it is validated for western blot detection of the UAP1 target in human, mouse, and rat samples. Polyclonal preparations recognise multiple epitopes across the antigen, which can help preserve signal when probing denatured lysates or when working with conserved regions of the protein. The IgG isotype and unconjugated format leave labelling choices to the end user, supporting downstream coupling to HRP, biotin, or a fluorophore of preference. UAP1 expression is investigated in cancer cell metabolism, nutrient sensing through O-GlcNAc modification, metabolic disease models, and bacterial cell wall mimicry pathways in pathogens such as Streptococcus, giving the antibody utility across glycobiology and host-microbe research. Researchers can compare UAP1 protein levels across tissues, cell lines, or treatment conditions, and the polyclonal format is often suited to tracking changes in total UAP1 abundance rather than isoform-specific events. For liquid IgG preparations, aliquoting upon receipt and limiting repeated freeze-thaw cycles helps preserve binding activity over long-term storage.