Anti-U2AF1 Antibody

Rabbit polyclonal antibody raised against U2AF1, the U2 small nuclear RNA auxiliary factor that recognizes the AG dinucleotide at the 3' splice site and is required for pre-mRNA splicing. This unconjugated reagent from Antibodies.com is supplied as 100 µl of polyclonal serum and supports detection of endogenous U2AF1 in both Western blot and immunohistochemistry applications, with verified reactivity toward human, mouse, and rat samples.

The antibody is widely used to study spliceosome assembly, alternative splicing regulation, and the oncogenic consequences of recurrent U2AF1 mutations, particularly the S34F and Q157P/Q157R hotspots that characterize myelodysplastic syndromes, secondary acute myeloid leukemia, lung adenocarcinoma, and other solid tumors. Researchers employ it to probe splicing factor expression in whole cell lysates, to localize U2AF1 in fixed tissue sections, and to investigate how altered 3' splice site recognition reshapes the transcriptome in hematologic malignancies. For research use only.