Anti-TYSY Antibody

Offered by Antibodies.com, this is a rabbit polyclonal antibody of the IgG isotype, supplied in unconjugated form. It is affinity purified and reacts with human samples. The immunogen is a synthetic peptide, and the antibody is provided in PBS buffer containing 0.09% sodium azide and 50% glycerol at pH 7.3. Recommended storage is at -20 °C, with freeze/thaw cycles to be avoided.

Third-party product documentation from Abclonal identifies the corresponding target as cGAS (synonyms: MB21D1, h-cGAS, C6orf150), a protein reported to possess 2',3'-cyclic GMP-AMP synthase, chromatin binding and phosphatidylinositol-4,5-bisphosphate binding activities, and which is found in the cytosol, the nucleus and at sites of double-strand break. Observed molecular weight by Western blot is 62 kDa; calculated molecular weight is 59 kDa. The Antibodies.com catalogue page lists the target as TYSY.

Recommended applications per the Abclonal literature are Western blot (1:500 – 1:4000), IHC-P (1:50 – 1:200), IF/ICC (1:50 – 1:200) and ELISA (suggested starting concentration 1 μg/mL). The Antibodies.com product page instead lists WB, IHC, IF and FC. Validation data referenced in the third-party documentation include Western blots of HeLa, HT-29 and THP-1 lysates, immunohistochemistry on paraffin-embedded human colon and colon carcinoma tissue, and immunofluorescence on U2OS cells.