Anti-TYR Antibody

Tyrosinase (TYR) is a copper-dependent, membrane-bound glycoprotein that serves as the rate-limiting enzyme in melanin biosynthesis, catalyzing the hydroxylation of L-tyrosine to L-DOPA and the subsequent oxidation to dopaquinone. This unconjugated rabbit polyclonal reagent from Antibodies.com targets the human, mouse, and rat TYR protein and is supplied as 100 µl of a 1 mg/ml stock in PBS buffer with 0.1% sodium azide and 50% glycerol as a cryoprotectant. It is validated for western blot, immunohistochemistry, and immunofluorescence workflows, supporting pigmentation studies, melanocyte biology, melanoma cell-line characterization, and investigations of oculocutaneous albinism type 1 (OCA1) and related TYR loss-of-function phenotypes. Researchers commonly deploy it to confirm expression of TYR in pigmented tissues such as skin and uveal melanocytes, to track melanogenic differentiation in cultured melanocytes or melanoma models, and to localize TYR immunoreactivity in fixed tissue sections. The polyclonal format offers an epitope-spread advantage for detecting multiple regions of the target, while the unconjugated presentation allows flexible pairing with user-selected secondary antibodies and fluorophores or HRP reporters. Store the vial refrigerated; avoid repeated freeze-thaw cycles to preserve antibody activity, and aliquot upon first use if long-term storage is needed. This is a research-use-only reagent.