Anti-TWIST1 Antibody

Produced in rabbits and affinity-purified, this unconjugated polyclonal IgG reagent targets TWIST1 (Twist Family BHLH Transcription Factor 1), a basic helix-loop-helix DNA-binding protein that drives epithelial-mesenchymal transition in development and disease. Antibodies.com supplies the antibody as a 100 µl liquid vial validated for western blot detection of endogenous TWIST1 in human, mouse, and rat lysates. Researchers deploy it to track metastatic progression in breast, prostate, and pancreatic cancer models, where TWIST1-induced EMT contributes to invasion and therapy resistance; to follow neural crest cell migration during vertebrate embryogenesis; and to characterize Saethre-Chotzen syndrome and related craniofacial malformations arising from TWIST1 haploinsufficiency. The reagent is applied to cell culture lysates, xenograft tumor homogenates, and embryonic tissue extracts, and it is used downstream with HRP-conjugated secondary antibodies for chemiluminescent visualization on standard SDS-PAGE gels, where the target resolves as a band near 25 kDa.