Anti-TWEAK Antibody

This rabbit polyclonal reagent from Antibodies.com is raised against TWEAK (TNFSF12), a member of the TNF ligand superfamily that signals through the Fn14 receptor (TNFRSF12A) to regulate inflammation, angiogenesis, and cell survival. The unconjugated antibody is supplied in a 100 µl vial and is validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows across human and mouse sample types.

TWEAK is expressed as a type II transmembrane protein that is proteolytically cleaved into a soluble ~18 kDa cytokine, and detection reagents must recognize both forms to support studies of autocrine and paracrine signaling. Polyclonal antibodies raised against the full-length ligand are useful in tissue-section imaging where the cleaved form is captured along with membrane-bound ligand in adjacent cells. The reagent is applicable to investigations of acute kidney injury, neuroinflammation, skeletal muscle regeneration, and tumor microenvironment remodeling, where TWEAK/Fn14 signaling has been linked to disease progression in published studies.

For storage and handling, aliquot upon first thaw to limit freeze-thaw cycles, refrigerate at 4 °C for routine use over several weeks, and hold frozen at -20 °C or colder for longer retention. The unconjugated format lets researchers pair the reagent with HRP, biotin, or fluorophore conjugates of their choice, supporting flexible assay design in cell culture experiments and in vivo tissue analysis.