Anti-Tubulin α Antibody

Alpha-tubulin is a ~50 kDa globular protein that polymerizes with beta-tubulin to form microtubules, the cytoskeletal filaments responsible for maintaining cell shape, supporting intracellular transport, and segregating chromosomes during mitosis. Antibodies.com offers this rabbit polyclonal reagent for researchers investigating cytoskeletal architecture, post-translational tubulin modifications (acetylation, tyrosination, polyglutamylation), and general protein loading normalization across immunoblotting experiments.

The unconjugated antibody is supplied in a 100 µL volume and is validated for western blot and ELISA workflows. It cross-reacts with human, mouse, and rat samples, making it broadly applicable to studies in cell lines, primary cultures, and tissue lysates. On SDS-PAGE, the target resolves as a band near 50-55 kDa, consistent with the apparent molecular weight of alpha-tubulin in most mammalian systems.

Common laboratory uses include normalization of lane loading, validation of fractionation purity when separating cytosolic and microtubule-associated pools, and characterization of microtubule dynamics in response to drug treatments or genetic perturbations. The polyclonal format allows recognition of multiple epitopes across the protein, which is useful when detecting post-translationally modified or partially processed forms of the target.