Anti-TSC2 (phospho-T1462) Antibody

Raised in rabbit against a phosphorylated epitope at threonine 1462 of tuberin (TSC2), this unconjugated polyclonal reagent from Antibodies.com supports Western blot detection of the activated tumor suppressor in human, mouse, and rat samples. The T1462 phospho-site sits within a regulatory region of the TSC1-TSC2 complex that is modulated by ERK-family kinases, and probing it allows researchers to follow input-output relationships of the mTOR signaling cascade under growth-factor, cell-cycle, or stress conditions. Each vial contains 100 µl of antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.3, ready to dilute directly into blocking buffer for immunoblotting of whole-cell or tissue lysates. Verified reactivity with mouse and rat orthologs extends the reagent to comparative rodent studies of tuberous sclerosis complex and metabolic disease, while the rabbit host and polyclonal format provide broad epitope coverage around the modified threonine residue even when target expression is low. The supplied 100 µl volume supports multiple probing rounds, membrane stripping, and re-blotting for time-course or treatment comparisons.