Anti-Trx Tag Antibody

The Thioredoxin (Trx) tag is a widely used fusion partner for recombinant protein expression in E. coli, where it promotes proper folding and enhances solubility of difficult target proteins. This unconjugated mouse polyclonal IgG antibody from Antibodies.com recognizes the Trx epitope and is formatted for Western blot detection of Trx-tagged constructs in cell lysates, bacterial extracts, and purified protein samples.

Produced in mouse hosts and supplied as 100 µl of polyclonal antibody, the reagent supports routine detection workflows in research laboratories that express thioredoxin-tagged fusion proteins. Because the target is an exogenous fusion tag rather than an endogenous host protein, the antibody reports only on samples engineered to carry the Trx fusion, making it a useful confirmation tool after cloning, expression screening, and purification steps. Researchers commonly apply this reagent to verify in-frame insertion, monitor expression levels across induction conditions, and track the fusion partner through affinity purification workflows.