Anti-TRPV4 Antibody

Manufactured by Antibodies.com, this primary antibody is a rabbit polyclonal reagent raised against the Transient Receptor Potential Vanilloid 4 (TRPV4) ion channel. It was generated using a recombinant human TRPV4 immunogen and subsequently purified by affinity chromatography on the immunogen, yielding a highly specific reagent that recognizes the endogenous total TRPV4 protein in native samples. The antibody is supplied at 1.0 mg/mL in a phosphate-buffered saline formulation (pH 7.4, 150 mM NaCl) containing no magnesium or calcium ions, supplemented with 0.02% sodium azide as a preservative and 50% glycerol for cryoprotection. The unconjugated format allows researchers flexibility to pair the antibody with custom labels of their choice. Validated applications include Western blotting (WB) and immunohistochemistry (IHC), and the reagent has been verified to cross-react with human, mouse, and rat TRPV4, making it well suited for comparative studies across these species. Each pack size of 50 µL is sufficient for multiple experiments and should be stored at −20 °C to preserve activity across freeze-thaw cycles. With its polyclonal profile, this reagent offers broad epitope coverage of TRPV4, increasing the likelihood of robust signal even if target expression is low or partially compromised in a given sample preparation.