Anti-Troponin I (13-22) Antibody

Cardiac Troponin I (cTnI) can be distinguished from skeletal isoforms using this mouse monoclonal antibody from Antibodies.com, which targets the N-terminal epitope spanning residues 13-22 of cTnI. The recognized region lies within the cardiac-specific N-terminal extension that is unique to the cardiac isoform, supporting isoform-specific detection in experimental research applications.

Purified via Protein A chromatography, the reagent is supplied as an unconjugated IgG3 isotype preparation at 1 mg per vial. It is validated for Western Blot (WB) detection of Troponin I in cardiac tissue lysates and cell-based samples. The unconjugated format offers flexibility to pair with a range of secondary antibodies and custom detection chemistries, including HRP, alkaline phosphatase, or fluorescent labels.

Key technical note: the N-terminal region spanning residues 13-22 is known to be susceptible to proteolytic cleavage in circulating serum, where endogenous proteases can truncate cTnI. For this reason, this antibody is best suited to analyzing freshly prepared tissue homogenates or cell lysates, where cTnI integrity is preserved, rather than serum specimens where degradation can compromise the signal.

Storage: Reconstitute in an appropriate buffer and store at -20 °C for long-term use. Aliquot to limit freeze-thaw cycles and protect from light to preserve antibody activity. Each vial supports multiple Western Blot experiments depending on dilution factor and membrane size.

Applications: Useful for cardiac injury research, myocardial tissue characterization, and comparative studies aimed at distinguishing cardiac from skeletal Troponin I isoforms. The monoclonal nature of the reagent provides batch-to-batch consistency and reproducible epitope-specific recognition across experiments.