Anti-TRIM5 Antibody

Manufactured by Antibodies.com as a polyclonal primary reagent, this rabbit-derived antibody targets TRIM5 (also known as RNF88/TRIM5alpha), a tripartite motif-containing protein of interest in innate immunity and retroviral restriction research. The antibody is supplied as a 50 µl liquid aliquot at a working concentration of 1 mg/ml, formulated in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, and is unconjugated, allowing it to be paired with the user's preferred secondary detection reagent. Because it is a polyclonal reagent raised in rabbit, the IgG preparation recognizes multiple epitopes on TRIM5, which can be advantageous for detecting the protein under varied sample conditions. The Antibodies.com product is validated for Western blot (WB) and immunohistochemistry (IHC) applications and has been tested for cross-reactivity across human, mouse, and rat samples, making it suitable for comparative studies in cell lines as well as in rodent tissue sections. Recommended storage is at -20 °C (or lower for long-term use) in the supplied glycerol-containing buffer to preserve activity and prevent repeated freeze-thaw degradation. This reagent is intended for research use only and is well suited for laboratories investigating TRIM5 expression patterns, post-translational regulation, and its role in restricting retroviral replication.