Anti-TRIM28 (C-terminus) Antibody

TRIM28 (Tripartite Motif Containing 28), also known as TIF1-beta or KAP1, operates as a transcriptional co-repressor that organizes repressive chromatin by recruiting SETDB1, HP1, and the NuRD complex, and it functions as a central node in the ATM-mediated DNA damage response through phosphorylation at Ser824. This unconjugated mouse monoclonal antibody from Antibodies.com targets the C-terminal region of human TRIM28 and is supplied as a 100 µL purified preparation in 0.1 M Tris-Glycine (pH 7.4) buffer containing 150 mM NaCl, 50% glycerol, and 0.2% sodium azide as a preservative.

Validated applications on human samples include Western blot, immunohistochemistry, and immunocytochemistry, supporting studies of KRAB-zinc finger-mediated gene silencing, retroelement repression, and chromatin remodeling in cancer biology and pluripotent stem cell research. Store at -20°C in working aliquots to limit freeze-thaw cycles; the 50% glycerol cryoprotectant allows the reagent to remain liquid at this temperature. The host is mouse, the clonality is monoclonal, and the conjugate is unconjugated, leaving the choice of secondary label and detection chemistry to the end user.