Anti-TRIM10 Antibody

Produced by Antibodies.com as a 100 µl liquid aliquot of antigen-affinity-purified rabbit IgG at 2.1 mg/mL, this polyclonal reagent binds endogenous human tripartite motif-containing protein 10 (TRIM10) through an immunogen raised against residues near the C-terminal of the protein. Supplied unconjugated in PBS (pH 7.4) with 40% glycerol and 0.05% sodium azide as preservative, the vial is shipped at 4°C and should be aliquoted and stored at -20°C to limit freeze-thaw cycles.

Western blot validation was performed on 40 µg of HeLa whole-cell lysate resolved on an 8% SDS-PAGE gel using a primary antibody dilution of 1/800 and a 30-second exposure time. Immunohistochemistry was validated on paraffin-embedded sections of human liver cancer and human breast cancer tissue at a working dilution of 1/35. Catalogued as catalogue A37354, the antibody detects total TRIM10 protein and is used by researchers characterising this E3 ubiquitin ligase within RIG-I-like receptor signalling, innate immune pathway regulation, and post-translational modification studies in human hematopoietic lineages.