Anti-TRDMT1 Antibody

This rabbit polyclonal IgG antibody recognizes TRDMT1, the tRNA aspartic acid methyltransferase 1 enzyme also known as DNMT2. Supplied by Antibodies.com as an unconjugated reagent in a 200 µl volume, the antibody was raised against the human TRDMT1 protein and was validated for Western blot and immunohistochemistry workflows. Cross-reactivity has been demonstrated on mouse tissue, supporting dual-species experimental designs that compare human cell line results with murine knockout or disease models.

TRDMT1 catalyzes the methylation of cytosine-38 within the anticodon loop of tRNA(Asp), an RNA modification linked to codon-specific translation fidelity, stress-granule assembly, and the biogenesis of tRNA-derived fragments that contribute to innate immune signaling. Researchers use anti-TRDMT1 reagents to probe how loss of this conserved methyltransferase affects differentiation, viral response, and the broader DNA-methylation-like enzyme network. The polyclonal format provides multiple epitope coverage, which is helpful when detecting endogenous expression levels that are often modest in primary cells.

Recommended applications include Western blot for confirming expression across tissue lysates and immunohistochemistry on formalin-fixed paraffin-embedded sections to map cellular localization. Store the vial refrigerated; avoid repeated freeze-thaw cycles and aliquot upon first use to preserve activity across long studies.