Anti-Transcription intermediary factor 1-beta Antibody

TRIM28, also called Transcription intermediary factor 1-beta (TIF1-beta) or KAP1, functions as a transcriptional corepressor that mediates KRAB-zinc finger protein silencing and orchestrates chromatin remodeling at target loci. This unconjugated rabbit polyclonal reagent from Antibodies.com was raised against TIF1-beta and is supplied as 100 µg of purified antibody, used in western blot and immunohistochemistry workflows on human samples.

In western blot, the antibody recognizes the endogenous ~110 kDa band characteristic of full-length TRIM28, enabling studies of DNA damage response signaling where phosphorylation at Ser824 serves as a kinetic marker of ATM-dependent activation. For immunohistochemistry, the reagent supports localization studies in formalin-fixed paraffin-embedded tissues, where TIF1-beta accumulates at pericentromeric heterochromatin and DNA double-strand break foci.

Researchers working with this antibody investigate KAP1-mediated silencing of endogenous retroviruses, pluripotency maintenance in embryonic stem cells, and the cross-talk between sumoylation and phosphorylation events that govern its repressor-to-activator switch. The unconjugated format allows flexible pairing with secondary antibodies labeled with HRP, biotin, or fluorophores to match individual detection chemistries. Storage at -20 °C in working aliquots preserves activity across multiple freeze-thaw cycles.